IMPROVEMENT IN ISOLATION AND AXENIC GROWTH OF GIARDIA LAMBLIA IN TYI-S-33 MEDIUM SUPPLEMENTED WITH BILE
S.A. TALAR *
DEPARTMENT OF PARASITOLOGY, KASHAN UNIVERSITY OF MEDICAL SCIENCES, KASHAN-IRAN.
M.R. TALARI *
DEPTT OF MICROBIOLOGY, ISLAMIC AZAD UNIVERSITY OF QOM, QOM-IRAN
M. ASMAR *
DEPTT OF PARASITOLOGY, PASTEURE INSTITUTE OF IRAN, TEHRAN-IRAN
*Author to whom correspondence should be addressed.
Abstract
total of 60 fresh Giardia lamblia cyst present in human fecal specimens isolated in Central Laboratory of shan University of Medical Sciences. Thirty-eight new isolates of G. lamblia was gradually adapted to as in TYI-S-33 medium supplemented with bile. The parasites grew luxuriantly and formed a monolayer the surface of the culture vessels. When G. lamblia was inoculated into to fresh medium, the population of separasites grew from 0.9 x 105 to 1.6 x 105 /ml in six days with a generation time of 12 to 13 hrs at 37°C.