Enriched Live Feed Quality of Artemia nauplii with Probiotic Lactiplantibacillus plantarum: Implications for Larval Health and Vibrio Resistance in Aquaculture
K. Vasuki
PG & Research Department of Zoology and Biotechnology, AVVM Sri Pushpam College (An Autonomous Institution, Affiliated to Bharathidasan University), Poondi, Thanjavur - 613503, Tamil Nadu, India.
K. C. Pravitha
PG & Research Department of Zoology and Biotechnology, AVVM Sri Pushpam College (An Autonomous Institution, Affiliated to Bharathidasan University), Poondi, Thanjavur - 613503, Tamil Nadu, India.
R. Murugesan
Division of Entomology, Department of Bio Sciences, Rajagiri College of Social Sciences (An Autonomous Institution, Affiliated to Mahatma Gandhi University), Rajagiri P.O., Kalamassery, Cochin – 683 104, Kerala, India.
B. Kaleeswaran *
PG & Research Department of Zoology and Biotechnology, AVVM Sri Pushpam College (An Autonomous Institution, Affiliated to Bharathidasan University), Poondi, Thanjavur - 613503, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
This study assesses the enrichment of Artemia nauplii with the probiotic Lactiplantibacillus plantarum (NCBI GenBank Accession No: PQ764068) to improve live feed quality in aquaculture. Newly hatched A. nauplii was exposed to bioencapsulation of probiotic L. plantarum concentrations from 106 to 108 CFU/mL for 24h, 48h, 72h, and 144h under laboratory conditions. The bioencapsulation efficiency was assessed in A. nauplii through survival, behavioral responses, in which the enhanced swimming activity, feeding behaviour, behavioural stress and better survival were observed in the highest probiotic enrichment (107 CFU/mL) load. The challenge study with Vibrio parahaemolyticus against the bioencapsulated A. nauplii showed better results in 108 CFU/mL than the positive and negative control groups. This research demonstrated that probiotic L. plantarum enriched A. nauplii can serve as an efficient live feed for carrying microbes to aquatic larvae. This will improve the growth and health of larvae and increase resistance against diseases in aquaculture practices.
Keywords: Aquaculture, Artemia nauplii, Lactiplantibacillus plantarum, Vibrio, Probiotic, survival, toxicity, behavioural activity