Evaluation of the Cytotoxic Potential of Lippia nodiflora (L.) Michx. against Mouse Adenocarcinoma in Swiss Albino Mice
Kadhiravan Murugaiah
PG and Research Department of Biochemistry, Srimad Andavan Arts and Science College (Autonomous), Affiliated to Bharathidasan University, Tiruchirappalli - 620 005, Tamil Nadu, India.
Kanimozhi Dharmaraj
PG and Research Department of Biochemistry, Srimad Andavan Arts and Science College (Autonomous), Affiliated to Bharathidasan University, Tiruchirappalli - 620 005, Tamil Nadu, India.
Agnel Arul John Nayagam
PG and Research Department of Biochemistry, Srimad Andavan Arts and Science College (Autonomous), Affiliated to Bharathidasan University, Tiruchirappalli - 620 005, Tamil Nadu, India.
Sridharan Gurunagarajan *
PG and Research Department of Biochemistry, Srimad Andavan Arts and Science College (Autonomous), Affiliated to Bharathidasan University, Tiruchirappalli - 620 005, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Objective: Lippia nodiflora (L.) Michx. (Verbenaceae) has been used in indigenous medicine and claimed to possess a wide range of biological activities, including anticancer activity. The present study was designed to assess the in-vitro and in-vivo anticancer effect of Lippia nodiflora (L) Michx on the EAC cell line.
Methods: EAC tumour-bearing mice were used to study anticancer activity. Monitoring the mean survival time and the impact on glycoproteins, antioxidant enzymes, and haematological and biochemical parameters were used to access the activity.
Result: The in vitro cytotoxicity against EAC cell lines, the EELN was effective. At the studied dose levels (100, 200, and 300 mg/kg bw), EELN had a strong effect on recovering hemopoietic system and considerably (*P=0.05) extended the mean survival time. The liver marker enzymes and antioxidants were recovered on EELN administration. Results from the data generated were similar to those from animals given with standard medication 5-fluorouracil.
Conclusion: The current study concluded that EELN has considerable increase in antioxidant system and inhibition of EAC growth in experimental animal.
Keywords: “Ethanol extract of Lippia nodiflora (L.) Michx” (EELN), “Ehrlich Ascites Carcinoma cell lines” (EAC), increase in life span (ILS), tumour growth response, glycoproteins