Dose-dependent Biopesticidal Activity of Annona squamosa against Sitophilus oryzae in Stored Sorghum Grain
Puja Singh *
Department of Zoology, A.N.D.N.N.M.M, P.P.N.(P.G.) College, Kanpur, India.
Sangeeta Avasthi
Department of Zoology, A.N.D.N.N.M.M, P.P.N.(P.G.) College, Kanpur, India.
Pushpa Choudhary
Department of Zoology, A.N.D.N.N.M.M, P.P.N.(P.G.) College, Kanpur, India.
Ritikesh Kumar Roy
Department of Zoology, A.N.D.N.N.M.M, P.P.N.(P.G.) College, Kanpur, India.
*Author to whom correspondence should be addressed.
Abstract
This study aimed to evaluate the dose-dependent biopesticidal activity of Annona squamosa seed extract against Sitophilus oryzae (rice weevil) in stored Sorghum grains under laboratory conditions. The experiment was conducted for a total duration of 45 days, during which the efficacy of three concentrations (5 g, 10 g, and 15 g per kg of grains) was assessed. Insect mortality was recorded at regular intervals throughout the 45-day experimental period. Results showed a clear dose and time-dependent response: The highest dose (15 g) achieved 100% mortality by day 10, the intermediate dose (10 g) by day 15, and the lowest dose (5 g) by day 15. There was no mortality seen in the untreated control. The results demonstrate that the extracts used are highly effective against insects and can serve as an eco-friendly alternative to synthetic pesticides. The replacement of synthetic chemical pesticides with plant-based alternatives is significant in reducing toxic residues, minimizing environmental contamination and promoting safer and sustainable grain storage practices. Annona squamosa extract was found to be significantly effective against the rice weevil and the study supports the integration of Annona squamosa into storage pest management strategies, contributing to reduced chemical residues, enhanced grain safety, and greater agricultural sustainability.
Keywords: Botanical extract, biopesticide, stored grain pest, dose-dependent mortality, sustainable pest management, climatic conditions