Genetic Characterization of Giant River Catfish, Sperata seenghala from River Sutlej Using Mitochondrial COI Marker
Supreet Kaur
College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India.
Grishma Tewari *
College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India.
Anuj Tyagi
College of Fisheries, Rani Lakshmi Bai Central Agricultural University, Jhansi-284003, Uttar Pradesh, India.
S. N. Datta
College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India.
Prabjeet Singh
Krishi Vigyan Kendra, Guru Angad Dev Veterinary and Animal Sciences University, Booh, Tarn Taran (Punjab), India.
*Author to whom correspondence should be addressed.
Abstract
The present study was aimed to generate baseline data on genetic stock structure of Giant River Catfish, Sperata seenghala from River Sutlej to formulate effective management and conservation measures for the concerned species. In this study, S. seenghala population from river Sutlej was characterized using mitochondrial cytochrome oxidase subunit I (COI) marker. A total of 26 numbers of raw COI sequences (>600 bp) obtained by Sanger sequencing showed nine haplotypes with haplotype diversity (Hd= 0.625), and nucleotide diversity (π=0.021), indicating moderate genetic variation in S. seenghala population. The phylogenetic analysis revealed that all COI sequences from Sutlej population were clustered in one clade showing the monophyletic origin of species. The present study revealed that mitochondrial COI sequences generated during the study clearly confirm the species as S. seenghala with moderate genetic variation in its population from river Sutlej in Punjab region of India. The data base generated during present study will be helpful to draft effective resource management and conservation strategies towards sustainable production of S. seenghala from river Sutlej.
Keywords: Catfish, genetic stock, mitochondrial COI, River Sutlej, Sperata seenghala