Effect of Essential Oil Supplementation on Gut Morphological Parameters of Japanese Quails Assessed by Histopathological Examination

Abhishek Tiwari *

Animal Nutrition Division, ICAR–National Dairy Research Institute (NDRI), Karnal–132001, Haryana, India.

Debashis Roy *

Department of Animal Nutrition, College of Veterinary & Animal Sciences, SVPUAT, Meerut - 250110, Uttar Pradesh, India.

Ajit Kumar

Department of Animal Nutrition, College of Veterinary & Animal Sciences, SVPUAT, Meerut - 250110, Uttar Pradesh, India.

Ram Kumar Singh

Department of Livestock Farm Complex (Animal Nutrition), College of Veterinary & Animal Sciences, SVPUAT, Meerut - 250110, Uttar Pradesh, India.

Vikas Jaiswal

Department of Veterinary Pathology, College of Veterinary & Animal Sciences, SVPUAT, Meerut-250110, Uttar Pradesh, India.

Manoj Kumar Singh

Department of Livestock Production Management, College of Veterinary & Animal Sciences, SVPUAT, Meerut-250110, Uttar Pradesh, India.

Harshit Verma

Department of Veterinary Microbiology, College of Veterinary & Animal Sciences, SVPUAT, Meerut-250110, Uttar Pradesh, India.

Shubham Nayak

Department of Animal Reproduction, Gynaecology and Obstetrics, ICAR-National Dairy Research Institute (NDRI), Karnal-132001, Haryana, India.

Kaushlendra Narayan Dwivedi

Department of Animal Nutrition, College of Veterinary & Animal Sciences, SVPUAT, Meerut - 250110, Uttar Pradesh, India.

Priyanka Rani

Department of Livestock Production Management, College of Veterinary & Animal Sciences, SVPUAT, Meerut-250110, Uttar Pradesh, India.

Rahul Tiwari

Division of Animal Nutrition, ICAR-National Dairy Research Institute (NDRI), Karnal-132001, Haryana, India.

Vaidehi Mishra

Department of Livestock Production Management, College of Veterinary & Animal Sciences, SVPUAT, Meerut-250110, Uttar Pradesh, India.

Gagan Chawla

Animal Physiology Division, ICAR-National Dairy Research Institute (NDRI), Karnal-132001, Haryana, India.

*Author to whom correspondence should be addressed.


Abstract

The present study was conducted to evaluate the effect of dietary supplementation of essential oil on growth performance, nutrient utilization of japanese quail birds. A total of two hundred (200), a day-old Japanese quail chicks were taken and divided randomly into five different treatment groups (T1, T 2, T3, T4 and T5) and every group was having four replicates having ten chicks in each. Diets were prepared by addition of cinnamon and peppermint essential oils in different concentrations, treatment group T1-basal or control diet for 35 days, T2-basal diet supplemented with cinnamon essential oil @500 ppm, T3-basal diet supplemented with peppermint essential oil @500 ppm, T4-basal diet supplemented with combination of cinnamon and peppermint essential oil @250 ppm each, T5 -Basal diet supplemented with combination of cinnamon and peppermint essential oil @ 500 ppm each. Gut histological parameters were found to be improved in all essential oil supplemented groups. Villus height (μm) was found higher (p<0.05) in essential oil supplemented groups than control. Crypt depth (μm) was found higher (p<0.05) in T2 and T3 group than control. It was concluded that dietary supplementation of cinnamon oil (@500ppm), peppermint oil (@500ppm) and combination of cinnamon and peppermint oil (@250 ppm each) significantly (p<0.05) improved gut morphological parameters, without adversely affecting blood biochemical parameters in Japanese quail birds.

Keywords: Cinnamon essential oil, Coturnix coturnix japonica, crypt depth, growth performance, gut morphology, japanese quail, nutrient utilization, peppermint essential oil, phytogenic feed additive, villus height


How to Cite

Tiwari, Abhishek, Debashis Roy, Ajit Kumar, Ram Kumar Singh, Vikas Jaiswal, Manoj Kumar Singh, Harshit Verma, et al. 2026. “Effect of Essential Oil Supplementation on Gut Morphological Parameters of Japanese Quails Assessed by Histopathological Examination”. UTTAR PRADESH JOURNAL OF ZOOLOGY 47 (4):107-15. https://doi.org/10.56557/upjoz/2026/v47i45516.

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