Immunomodulatory Effect of Nigella sativa Extract on Caprine Lymphocytes
R. S. Ghadge *
Veterinary Epidemiology and Preventive Medicine, NVC, MAFSU 440006, India.
C. G Panchbhai
Veterinary Epidemiology and Preventive Medicine, NVC, MAFSU 440006, India.
P.A.Tembhurne
Veterinary Microbiology and Biotechnology, NVC, MAFSU 440006, India.
*Author to whom correspondence should be addressed.
Abstract
Background: A lymphocyte proliferation assay (LPA) is a laboratory test used to evaluate the functional capability of lymphocytes by measuring their capacity to proliferate in response to an antigen, mitogen, or foreign molecule. Nigella sativa has immunomodulatory activity, which can affect or modify goats' immune systems. Studies have shown that it may strengthen the body's defences against different infections and advance general health by improving both innate and adaptive immune responses.
Aim: The present study evaluates the immunomodulatory effect of Nigella sativa extract on Caprine Lymphocytes.
Methods: The various bands of blood components in tubes were formed with plasma at the top followed by the buffy coat of lymphocytes, and the histopaque RBC of the bottom. The buffy coat was collected carefully of lymphocytes (between the upper plasma and lower RBC using a sterile serological pipette) without agitating the tube.
Results and Discussion: In the present investigation, the immunomodulatory effect of the ethanolic extract of Nigella sativa was analysed by the lymphocyte proliferation assay, performed with 6.25 ug/ml,12.5 ug/ml & 25 ug/ml of extract. The production of cytokines or nitric oxide, phagocytic and cytotoxic activities, proliferation of T cells and splenocytes, and vulnerability to necrosis and apoptosis in vitro and in vivo are all factors that support that NS affects immune cells.
Conclusion: The present study found that only 6.25 ug/ml of N. sativa showed lymphoproliferation, whereas none of the higher concentrations showed proliferation. The present study indicated that the lowest dilution,6.25 ug/ml has immunomodulatory activity.
Keywords: Lymphocytes, Nigella sativa, immunomodulation, necrosis