Study on Clinical Application of Exfoliative Vaginal Cytology in Canine Reproduction
K. S. Shelkar *
Department of Animal Reproduction, Gynaecology, Obstetrics, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Satara, India.
K. P. Khillare
Department of Animal Reproduction, Gynaecology, Obstetrics, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Satara, India.
A. B. Mali
Department of Animal Reproduction, Gynaecology, Obstetrics, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Satara, India.
M. N. Rangnekar
Department of Animal Reproduction, Gynaecology, Obstetrics, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Satara, India.
R. R Shelar
Department of Animal Reproduction, Gynaecology, Obstetrics, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Satara, India.
S. S. Chavan
Department of Animal Reproduction, Gynaecology, Obstetrics, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Satara, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Exfoliative vaginal cytology because of its simplicity, accessible equipment, and the possibility of getting rapid results could be used as a valuable technique to any reproductive diagnosis in she-dogs. The knowledge of the reproductive physiology of the estrous cycle is important for animal management and to determine the reproductive and productive potential of animals
Aim: The present study aimed to determine the ovulation time in canines.
Place and Duration of Study: Veterinary Clinical Complex, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Satara. September 4th, 2024 to February 26th, 2025.
Methodology: We studied 24 bitches through exfoliative vaginal cytology using Giemsa staining method.
Results: In total 24 bitches were studied out of which, natural mating was carried out in 21 bitches whereas in three bitches, artificial insemination with fresh semen was done. Twenty out of 24 bitches were found pregnant upon transabdominal ultrasound one month after mating or artificial insemination. Ultrasonography was conducted one month after the final day of mating to identify early pregnancy through ultrasonographic assessment of the gestational sac, along with the body and head of the fetus in the pregnant bitches. Fetal heart activity was evaluated through ultrasonography, demonstrating the fetus's viability. Additionally, non-pregnancies were verified through ultrasonographic assessment.
Conclusion: Vaginal cytology increases the conception rates in canine by giving an ideal time for mating.
Keywords: Exfoliative vaginal cytology, dog, conception, canine