Isolation and Characterization of Collagen from Cephalothorax of Penaeus
Jayanthi J
*
Department of Advanced Zoology and Biotechnology, Guru Nanak College (Autonomous), Guru Nanak Salai, Velachery, Chennai – 600042, Tamil Nadu, India.
Jevedha M
Department of Advanced Zoology and Biotechnology, Guru Nanak College (Autonomous), Guru Nanak Salai, Velachery, Chennai – 600042, Tamil Nadu, India.
Ragunathan M.G
Department of Advanced Zoology and Biotechnology, Guru Nanak College (Autonomous), Guru Nanak Salai, Velachery, Chennai – 600042, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
This study aimed to examine the yield and properties of collagen protein isolated from the cephalothorax of Penaeus monodon. Collagen underwent analysis using gel electrophoresis. The results indicated that collagen comprises α1 and α2 subunits with corresponding average molecular weights. The investigation into collagen solubility revealed that, in the presence of NaCl, collagen exhibited the greatest relative solubility at 2% NaCl (80.20 ± 4.95%). Conversely, solubility diminished with elevated NaCl concentrations. Collagen had the maximum relative solubility at pH 3 (91.32 ± 5.14%), with solubility diminishing at elevated pH levels. FT-IR spectroscopy was employed to contrast collagen with a model molecule. Five wavenumbers in the spectrum for model collagen were identified: Amide A (3,406–3,421 cm⁻¹), Amide B (2,916–2,940 cm⁻¹), Amide I (1,639–1,640 cm⁻¹), Amide II (1,539–1,570 cm⁻¹), and Amide III (1,234–1,250 cm⁻¹). The cytotoxic impact on 3T3-L1 cells was evaluated.
Keywords: Collagen, prawn, SDS-PAGE, FT-IR spectroscopy