Cellulase Production from Bacillus subtilis CAS PG2 Using Lignocellulosic Wastes: A Statistical Approach
Prabakaran Ganesan *
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608502, Tamil Nadu, India.
MayavuPackiyam
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608502, Tamil Nadu, India.
VijayanandPackiyaraj
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608502, Tamil Nadu, India.
Gnanakkan Ananthan
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608502, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
The cellulase producing bacterium Bacillus subtilis CAS PG2 was isolated from fish gut of estuarine common carp (Manakkudi Estuary, Kanyakumari District, Tamil Nadu, India) and identified through 16S rRNA gene sequences. Optimization of medium components and culture conditions for cellulase production were optimized by response surface methodology using cheap raw material such as Paper Industry Waste (PIW), Tamarind Kernel Powder (TKP), Palm Jaggery (PJ) and Rice Bran (RB). Plackett-Burman tool was used to determine the ideal medium ingredients and culture conditions for increased cellulase production. Utilizing central composite design, the optimal medium components for achieving maximum cellulase production (1124.29 U/mL) were determined as follows: rice bran (7.50 g/L), peptone (5.00 g/L), KH2PO4(0.75 g/L), and NaCl (7.50 g/L). Thus, the cellulase from fish gut-associated Bacillus subtilis CAS PG2 that uses lignocellulosic biomass could be quite valuable in developing industrial value added products.
Keywords: Bacillus subtilis, cellulosic wastes, cellulase, lignocellulosic wastes