UTTAR PRADESH JOURNAL OF ZOOLOGY

Titanium dioxide is an extremely active in a variety of reactions, nontoxic, and reasonably priced and it also used extensively in water purification processes such as photocatalysis, catalysis, and dye degradation. Titanium dioxide nanoparticles (TiO₂ NPs) potent oxidizing ability through the production of free radicals promotes the inactivation of bacteria by reducing their development. TiO₂ NPs, using the sol-gel method, titanium precursors are combined with isopropanol, deionized water, and acids to create titanium dioxide nanoparticles. The present study focused on characterization and evaluation of synthetic TiO₂ NPs for its anti-inflammatory, antioxidant and antimicrobial ability. Anti-inflammatory activity tested using bovine serum albumin/anti-denaturation assay, In-vitro antioxidant assay by (DPPH) and antibacterial activity by Agar well diffusion method was also performed. At a concentration of 100 µg/ml, the results showed that chemically produced TiO₂ NPs have the ability to scavenge the free radical DPPH, neutralizing 72% of the radicals. It was shown that a sample of 100 µl of TiO₂ NPs had strong anti-inflammatory properties and inhibited 69% of protein denaturation. TiO₂ NPs were tested against pathogenic bacterial strains at varying concentrations (50, 75, and 100 mg/ml), they demonstrated significant antibacterial activity by inhibiting both Gram positive and Gram-negative bacteria. In the present study the prepared TiO₂ NPs showed potent water purification property in the artificial polluted water.