Study on the Effect of B. Serrata and Bovine Follicular Fluid on Hydroxyproline Content in Achilles tendon Defect in Dogs
Ahmed F. Jaber Al-Asady *
Department of Surgery and Obstetrics, Veterinary Medicine College, Basrah University, Basrah, Iraq and Dhi-Qar Veterinary Teaching Hospital, Veterinary Directorate, Dhi-Qar, Iraq.
Alaa Ahmed Ibrahim
Department of Surgery and Obstetrics, Veterinary Medicine College, Basrah University, Basrah, Iraq.
Ihsan. A. Habeeb
Department of Surgery and Obstetrics, Veterinary Medicine College, Basrah University, Basrah, Iraq.
*Author to whom correspondence should be addressed.
Abstract
Background: Calcaneal tendon (Achilles tendon) consists of multiple tendons from several muscles of the posterior limb and represent as strongest tendon in body. Traumatic injury is the most common affection of the Achilles tendon which may cause tendon damage lead the foot to fall flat – much like humans walk.
Objective: The present study is designed to compare the efficacy of B. Serrata extract and bovine follicular fluid on tendon healing and regeneration process in dog.
Material and Methods: Twenty-seven adult local breed dogs, weighing 18-20 kg. Dogs were categorized into three equal groups (9 dogs), as control, treated 1 and treated 2. Surgical site was prepared under aseptic technique, After Achilles tendon exposure, tendon defect was induced as a core lesion in all dogs by penetrating tendon using 16-gauge needle. Tendon defect was treated with local application of autologous PRF in controls group. Tendon defect in treated group1 was treated via local usage of autologous PRF and B. Serrata extract. While in treated group 2 the tendon defect was treated by Autologous PRF and Bovine Follicular Fluid via local application. The surgical site was closed routinely and the animals were evaluated clinically, macroscopically, microscopically, and hydroxyproline test was performed along study period.
Results: Clinically, redness and swelling of the surgical site were less and disappeared in treated groups as a compare with control group. While lameness was significantly disappeared in B. Serrata group in 9 days' post-surgery when compared with bovine follicular fluid and control groups. Hydroxyproline content of Achilles tendon was significantly increase in B. serrata group as a compare with Bovine follicular fluid and control group after 30-day post-surgery. on the other hand, Bovine FF group exhibited a high level of hydroxyproline when contrast with control group at the same period.
Conclusion: B. serrata extract and bovine follicular fluid can enhance tendon healing and regeneration process but B. Serrata showed the most effective results in tendon rebuilding process.
Keywords: Hydroxyproline, Achilles tendon, B. Serrata, Bovine follicular fluid, Dog