Molecular Characterization of Rhipicephalus microplus Using Sanger Sequencing COI & 18S rRNA Markers from Chhatrapati Sambhajinagar, India
Sunita N. Borde
Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Chh. Sambhajinangar, India.
Sagar S. Gavali *
Department of Zoology, S.B.E.S College of Science, Chh. Sambhajinangar, India.
Arti Puri
Department of Zoology, Sant Dnyaneshwar College, Soygoan, India.
Sushil Jawle
Department of Zoology, Sant Dnyaneshwar College, Soygoan, India.
*Author to whom correspondence should be addressed.
Abstract
Genus Rhipicephalus ticks, which infest cattle, are the main animal parasites that cause billions of rupees' worth of annual economic losses. Species identification is highly challenging because of the physical similarities among the members of the Rhipicephalus (Boophilus) genus. The adult of R. microplus ticks from Chh. Sambhajinagar (MS) India, were examined morphologically and molecularly in this work. The morphology of the R. microplus isolates was different from that of the real R. microplus clade A ticks. Out of 13 sample of species 2 species analysed and successful sequencing outcome a by using sanger sequencing COI & 18S rRNA marker the database inserted into BLAST ncbi database and result matches with Rephicephalus microplus. Our study looked into tick diversity using a molecular technique that included phylogenetic analysis, DNA sequencing, and PCR.
Keywords: Rhipicephalus microplus, sanger sequencing, COI, 18s rRNA