Exploring Microbiological Quality Assessment Techniques and Antibiotic Sensitivity: A Comprehensive Review
Vidhya C.S.
Department of Primary Processing Storage and Handling, NIFTEM-Thanjavur, Thanjavur-613005, Tamil Nadu, India.
G. Gomadhi
Krishi Vigyan Kendra, Tindivanam, Villupuram District, Tamil Nadu-604 102, India.
Ningaraj Belagalla
Department of Entomology, Sampoorna International Institute of Agril. Science and Horticulture Technology, Mysore University, Belekere, India.
S. Jaya Prabhavathi *
Regional Research Station, Tamil Nadu Agricultural University, Vridhachalam,606 001, Cuddalore District, Tamil Nadu, India.
Jayeeta Majumder
Brainware University, Kolkata 700125, India.
Sourav Gangopadhyay
Brainware University, Kolkata 700125, India.
Susmi Biswas
Haldia Institute of Management (MAKAUT), India.
Himanshu Sekhar Behera
Department of Soil Science and Agricultural Chemistry, Lovely Professional University Punjab, India.
G. Malathi
Krishi Vigyan Kendra, Sandhiyur, Salem - 636 203, Tamil Nadu, India.
P. Kalaiselvi
ICAR- Krishi Vigyan Kendra, Sandhiyur, Salem-203, India.
*Author to whom correspondence should be addressed.
Abstract
Microbiological quality assessment and antibiotic sensitivity testing are crucial components of ensuring public health safety, food quality, and effective antimicrobial therapy and provides a comprehensive overview of the methodologies, challenges, and recent advancements in microbiological quality assessment and antibiotic sensitivity testing. It covers various techniques employed in microbiological quality assessment, including culture-based methods, molecular techniques, and rapid detection methods. Additionally, it explores the principles and methodologies of antibiotic sensitivity testing, focusing on disk diffusion, broth microdilution, and automated systems. Furthermore, the article discusses emerging trends and technologies in the field, such as whole-genome sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and understanding these techniques and their applications is essential for researchers, healthcare professionals, and policymakers to combat microbial infections effectively and safeguard public health.
Keywords: Microbiological quality assessment, antibiotic sensitivity testing, culture-based methods, molecular techniques, rapid detection methods, disk diffusion, broth microdilution, whole-genome sequencing, MALDI-TOF MS