HPTLC Analysis of Tephorisa pulicherrima (Baker) Gamble Ethanol Extract of Root
S. Anbukkarasi *
PG & Research Department of Chemistry, Nehru Memorial College (Autonomous), Puthanampatti, 621 007, Affiliated to Bharathidasan University, Tamil Nadu, India.
M. Ramesh
PG & Research Department of Chemistry, Nehru Memorial College (Autonomous), Puthanampatti, 621 007, Affiliated to Bharathidasan University, Tamil Nadu, India.
G. Revathi
PG & Research Department of Zoology, Nehru Memorial College (Autonomous), Puthanampatti, 621 007, Affiliated to Bharathidasan University Tamil Nadu, India.
V. Ramesh
PG & Research Department of Zoology, Nehru Memorial College (Autonomous), Puthanampatti, 621 007, Affiliated to Bharathidasan University Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Tephrosia pulcherrima commonly known as “Kalakkozhuppa,” is well known for its therapeutic properties in the Indian traditional medicinal system. The presence of important bioactive compounds in plants makes them pharmacologically valuable. Therefore, in this study, the High-Performance Thin Layer Chromatography (HPTLC) analysis of Tephrosia pulcherrima (root) ethanol extract was performed for its phytochemical profiling. The HPTLC densitometric analysis of the ethanol extract of Tephrosia pulcherrima was carried out using CAMAG HPTLC system, and the results were obtained in the form of chromatograms (scanned at the wavelength of 254 nm and 366 nm) representing several peaks. The phytochemical profile of the plant was determined and presented in the tables showing the total number of peaks, peak heights, peak area, percent area, and Rf values. The study concluded that T. pulcherrima ethanol extract of the root contains a rich variety of phytochemicals which might be accountable for its therapeutic value and thus justifies its traditional use in India.
Keywords: Tephrosia pulcherrima, ethanol extract, phtochemistry, HPTL, chromatogram