MODELING STRUCTURE DESIGN OF Mycobacterium tuberculosis KU- PROTEIN USING MODELER AS WELL STUDY INTERACTION BETWEEN KU-PROTEIN AND DNA POLYMERASE
SHASHANK RANA
Department of Microbiology, C. C. S. University Campus (UP), Meerut, India.
SAKSHAM KHUSHARIA
Department of Biotechnology, Noida International University, Gr. Noida (UP), India.
JYOTI PANDEY
Department of Biotechnology, Singhania University (Rajasthan), India.
NIKUNAJ BHARDWAJ *
Department of zoology, M. S. College, Saharanpur (UP), India.
*Author to whom correspondence should be addressed.
Abstract
Homology modeling is a significant computational procedure, in the field of structural biology. It is used for the determination of 3D structure of proteins. It practices accessible high-resolution protein constructions to develop a model of similar, but unidentified, structure. The purpose of that study was to explain the homology modeling as well as docking for the mycobacterium tuberculosis KU-protein (Rv0937) with diverse DNA polymerase I as ligands by with the help of CLUSPRO. KU-protein of Mycobacterium tuberculosis is an extremely conserved DNA binding protein. KU-protein sequence was selected as target molecule and further the template having determined identity and length near to the template was selected besides the 3D structure of the template(3com) was taken from the PDB (3com.pdb) and based on the template structure of the backbone of the target (mku) was formed and the target molecule was exhibited. The authentication of the protein modeled (mkutb) was done through the PROCHECK and protein-protein docking is completed by CLUSPRO, characterizes the first complete lyautomatic, web-based database for the docking of protein constructions and in silico screening and further to understand the structural basis of ligand binding.
Keywords: KU-protein, 3dstructure, CLUSPRO, ligand, PROCHECK