ANTIOXIDANT PROPERTIES OF TRASH FISH Odonus niger LIVER OIL IN COMPARISON WITH COD LIVER OIL
KARISAMY GOWRI GIRUBA
MNP Laboratory, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam-629502, Kanyakumari District, Tamil Nadu, India.
USHA RANI SARDHAR GOKULA DHARSINI
Department of Biotechnology, Udaya School of Engineering, Vellamodi, Kanyakumari District, Tamil Nadu, India.
GRASIAN IMMANUEL *
MNP Laboratory, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam-629502, Kanyakumari District, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
The present study was undertaken to compare the in vitro antioxidant properties of marine trash fish Odonus niger liver oil and commercially available cod liver oil. For this O. niger fishes were collected and the oil was extracted from the liver mass by following Bligh and Dyer method and it was purified. Further the antioxidant potential of various concentrations (12.5 to 200µg/ml) of the extracted oil was performed through different standard antioxidant assays, simultaneously the same concentrations of commercial cod liver oil were also performed. The antioxidant potential of both oils was compared with appropriate standards like ascorbic acid, gallic acid and quercetin in the respective assays. The different assays performed were hydroxyl radical scavenging activity (23.24%), total antioxidant activity (43.06%), DPPH radical scavenging activity (60.06%), reducing power effect (68.22%), lipid peroxidation inhibitory activity (53.10%) and ferrous ions chelating activity (75%) respectively for O. niger at the highest concentration of 200μg/ml. All the above activities showed that O. niger liver oil possessed better antioxidant potential, especially at higher concentration (200 µg/ml) than the tested commercial cod liver oil.
Keywords: Odonus niger, fish liver oil, antioxidant activity, cod liver oil