EVALUATION OF OXIDATIVE STRESS IN AZOXYMETHANE-INDUCED COLON CANCEROUS FISCHER RATS
V. KAVITHA
Department of Biotechnology, Krishna University, Machilipatnam, Andhra Pradesh, India.
B. BVIJAYALAKSHMI
Institute of Genetics, Osmania University, Hyderabad, Telangana, India.
J. NAVEENA LAVANYA LATHA *
Department of Biotechnology, Krishna University, Machilipatnam, Andhra Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Objective: To study the redox status of normal colon and aberrant crypts formed in azoxymethane induced colon cancerous fischer rats.
Methods: A total of 16 five-week-old male Fisher 344 rats (Rattus norvegicus), weighing approximately 90–100 grams were housed individually in plastic cages with wood-chip bedding. The animals were acclimatized for 1 week and fed with an American Institute of Nutrition (AIN-93G) diet ad libitum. Their protein oxidation, DNA damage, lipid peroxidation and antioxidants, glutathione (GSH), and antioxidative enzymes in serum were detected.
Results: The levels of protein oxidation and lipid peroxidation were significantly higher in the study group than in the control group (P<0.01). However, the mean serum level of MDA and conjugated diene was lower in the study group than in the control group (P<0.01). The activity of antioxidative enzymes was significantly decreased in the study group compared to control group (P<0.01).
Conclusion: Colorectal cancer is associated with oxidative stress, and assessment of oxidative stress and antioxidantsincorporation could be an important choice for the treatment and prevention of colorectal cancer.
Keywords: Colorectal cancerl azoxymethanel fischer ratsl oxidative stress, lipid peroxidation, protein oxidation