THE CHRONIC EFFECT OF FORCE-FEEDING (ORAL GAVAGE) ADMINISTRATION OF ASPARTAME ON WISTAR RATS (NEPHROTOXICITY AND NEUROBEHAVIORAL TROUBLES)
MABROUK BOUSSENA
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, Badji Mokhtar Annaba University, Algeria.
SAMIR DJEMLI *
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, Badji Mokhtar Annaba University, Algeria.
ABDELKRIM TAHRAOUI
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, Badji Mokhtar Annaba University, Algeria.
INES REFES
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, Badji Mokhtar Annaba University, Algeria.
FAIZA ROUAG
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, Badji Mokhtar Annaba University, Algeria.
SAMIA MESSIKH
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, Badji Mokhtar Annaba University, Algeria.
IBTISSEM CHOUBA
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, Badji Mokhtar Annaba University, Algeria.
ROUYA MEMOUNI
Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, Badji Mokhtar Annaba University, Algeria.
NADJETTE LAOUABDIA SELLAMI
Department of Biology, 08 May 1945 Guelma University, Algeria.
*Author to whom correspondence should be addressed.
Abstract
Background: This study was performed to see the effect of chronic administration by gavage of aspartame on kidneys and behavior in Wistar rats.
Methods: In this study, 28 Wistar rats were used, divided into 04 groups including a control group treated with distilled water and three other groups treated with aspartame at different doses (40 mg / kg, 60 mg / kg, 1000 mg / kg body weight) for 90 days orally (gastric gavage).
Results: A highly significant increase (p <0,001) of blood urea level ASP 3 group compared to the ASP1 group (ADI Acceptable Daily Intake) , however we observe a highly significant reduction in blood creatinine level (p <0, 01) as compared to the ASP1 tolerated daily intake group. In the open field test, we notice highly significant decrease (p <0,01) in the ASP3 group treated with the 1000 mg per kg body weight dose ASP 3 compared to the group treated with the 40 mg / kg bw dose or the ADI ( Acceptable Daily Intake) ASP1.
The histopathological study of kidneys showed multiple vascular congestion with inflammatory infiltration in Group ASP3 compared to the control.
Conclusion: The chronic administarion of aspartame by gavage on Wistar rats causes kidney damage and neurobehavioral troubles.
Keywords: Aspartame, kidneys, neurobehavioral, wistar, histopathological