HISTOCHEMICAL CHANGES IN THE INTESTINE OF Tilapia mossambica INDUCED BY ACUTE TOXICITY OF SODIUM FLUORIDE DURING SUMMER
M. B. BAGALE *
Department of Zoology, Sangameshwar College, Post Box No.52, Solapur-413001, Maharashtra, India.
K. R. RAO
Department of Zoology, Walchand College of Arts and Science, Solapur-413006, Maharashtra, India.
N. V. SHAH
Department of Zoology, Walchand College of Arts and Science, Solapur-413006, Maharashtra, India.
*Author to whom correspondence should be addressed.
Abstract
The objective of this study was to investigate the toxic effect of sodium fluoride (NaF) on freshwater fish, Tilapia (Oreochromis mossambica). Acute toxicity for 24, 48, 72 and 96 hrs. exposure of LC0 value was 30.0 ppm and LC50 value was 54.0 ppm for 96 hrs. The histochemical observation revealed that the neutral mucosubstances and glycogen content of intestine Tilapia mossambica showed a progressive decrease in staining intensity to Periodic acid Schiff (PAS) and Alcian blue pH-1 - Periodic acid Schiff (AB1- PAS) showed a progressive increase in purple blue staining showed increased acidic mucosubstances (sulfomucins) and decrease neutral mucosubstances and glycogen over time of exposure. The histochemical changes indicate sodium fluoride is toxic to growth and survival of the species.
Keywords: Fluoride, Tilapia mossambica, acute toxicity, histochemistry, intestine.