EFFECT OF TRYPSIN ON THE CHROMOCENTRIC MASS OF THE SALIVARY GLAND CHROMOSOMES OF DROSOPHILA MELANOGASTER, D. HYDEI, D. VIRLIS AND D. MIRANDA
CHANDRA SEKHAR CHAKRABARTI *
DEPARTMENT OF ZOOLOGY, NORTH BENGAL UNIVERSITY, RAJA RAMMOHANPUR, SILIGURI-734430, INIDIA.
*Author to whom correspondence should be addressed.
Abstract
Chromocentre of the polytene chromosome is a heterochromatin mass and is late replicating. It is very difficult to study the pattern of replication and transcription of the genetic material of this area without unmasking the protein-nucleic acid association of the chromocentre. In the present set of experiments attempt was made to digest some proteins of the chromocentre by trypsinization. Trypsin treated chromocenters of the four species of Drosophila studied showed different degrees of morphological changes.